CLONING AND EXPRESSION OF HORMOCONIS-RESINAE GLUCOAMYLASE-P CDNA IN SACCHAROMYCES-CEREVISIAE

被引：11

作者：

VAINIO, AEI

TORKKELI, HT

TUUSA, T

AHO, SA

FAGERSTROM, BR

KORHOLA, MP

机构：

[1] Research Laboratories, Alko Ltd, Helsinki, SF-00101

来源：

CURRENT GENETICS | 1993年 / 24卷 / 1-2期

关键词：

GLUCOAMYLASE; GENE CLONING; HORMOCONIS-RESINAE; SACCHAROMYCES-CEREVISIAE;

D O I：

10.1007/BF00324663

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A cDNA coding for glucoamylase P of Hormoconis resinae was cloned using a synthetic oligonucleotide probe coding for a peptide fragment of the purified enzyme and polyclonal anti-glucoamylase antibodies. Nucleotide-sequence analysis revealed an open reading frame of 1848 base pairs coding for a protein of 616 amino-acid residues. Comparison with other fungal glucoamylase amino-acid sequences showed homologies of 37-48%. The glucoamylase cDNA, when introduced into Saccharomyces cerevisiae under the control of the yeast ADC1 promoter, directed the secretion of active glucoamylase P into the growth medium.

引用

页码：38 / 44

页数：7

共 41 条

[1]

AMMERER G, 1983, METHOD ENZYMOL, V101, P192

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