RAPID ACQUISITION OF 3-DIMENSIONAL TRIPLE-RESONANCE EXPERIMENTS USING PULSED FIELD GRADIENT TECHNIQUES

被引：32

作者：

DAVIS, AL ^{[1
]}

BOELENS, R ^{[1
]}

KAPTEIN, R ^{[1
]}

机构：

[1] UNIV UTRECHT,BIJVOET CTR BIOMOLEC RES,DEPT CHEM,PADUALAAN 8,3584 CH UTRECHT,NETHERLANDS

来源：

JOURNAL OF BIOMOLECULAR NMR | 1992年 / 2卷 / 04期

关键词：

TRIPLE-RESONANCE; PULSED FIELD GRADIENTS; 3D NMR; PROTEINS;

D O I：

10.1007/BF01874817

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid method for recording three-dimensional triple-resonance experiments utilising pulsed field gradient techniques is proposed, and applied to the HNCO experiment. In order to optimise the sensitivity of the method, a short phase cycle is used in conjunction with the pulsed field gradients to select the desired coherence transfer pathway. The method is demonstrated for the HU protein.

引用

页码：395 / 400

页数：6

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