2 GENES ENCODING RIBOSOMAL-PROTEIN L3 OF SCHIZOSACCHAROMYCES-POMBE AND THEIR PROXIMAL PROMOTER REGIONS

被引：8

作者：

LIEBICH, I

KOHLER, G

WITT, I

GROSS, T

KAUFER, NF

机构：

[1] DREXEL UNIV,DEPT BIOSCI & BIOTECHNOL,PHILADELPHIA,PA 19104

[2] FREE UNIV BERLIN,INST BIOCHEM & MOLEK BIOL,W-1000 BERLIN 33,GERMANY

来源：

GENE | 1994年 / 142卷 / 01期

关键词：

RECOMBINANT DNA; SACCHAROMYCES CEREVISIAE; GENE COPIES; HOMOL D-ELEMENT; FISSION YEAST;

D O I：

10.1016/0378-1119(94)90365-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have cloned and sequenced two genes, rpl3-1 and rpl3-2, encoding the ribosomal protein L3 of Schizosaccharomyces pombe. The two genes contain an open reading frame encoding 388 amino acids (aa) with a M(r) of 43 808. The aa sequences are identical, except at position 78, where Rpl3-1 displays a valine residue and Rpl3-2 contains isoleucine. The aa sequences show 75% identity to the RPL3 aa sequence from Saccharomyces cerevisiae. S1-nuclease protection analysis revealed that both genes are transcribed. The promoter sequences of the two rpl3 genes are significantly different, but both promoters contain the conserved homol-D element. Transcription starts between 40 and 50 nt downstream from this element.

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页码：119 / 122

页数：4

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