INFLUENCE OF ANTIGEN PROPERTIES ON THE CONDITIONS REQUIRED TO ELUTE ANTIBODIES FROM IMMUNOADSORBENTS

Immunoadsorbents were used to purify a number of antibodies. Using pH 2.0 acid conditions alone it was possible to elute antibodies raised against human growth hormone (HGH) and Fc fragments from such immunoadsorbents with 50% or better recovery of antibody activity. However, to elute antibodies raised against triiodothyronine and cortisol required 6 M guanidine HCL, pH 2.0. The avidities of the purified antibodies were similar to those of the non-purified antibodies. The purified antibodies were stable in solution at 4°C for at least six months. © 1979.