DESIGN OF CAMP-CRP-ACTIVATED PROMOTERS IN ESCHERICHIA-COLI

被引：30

作者：

VALENTINHANSEN, P ^{[1
]}

HOLST, B ^{[1
]}

SOGAARDANDERSEN, L ^{[1
]}

MARTINUSSEN, J ^{[1
]}

NESVERA, J ^{[1
]}

DOUTHWAITE, SR ^{[1
]}

机构：

[1] CZECHOSLOVAK ACAD SCI, INST MICROBIOL, CS-14220 PRAGUE 4, CZECHOSLOVAKIA

来源：

MOLECULAR MICROBIOLOGY | 1991年 / 5卷 / 02期

关键词：

D O I：

10.1111/j.1365-2958.1991.tb02126.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have studied the deoP2 promoter of Escherichia coli to define features that are required for optimal activation by the complex of adenosine 3',5' monophosphate (cAMP) and the cAMP receptor protein (CRP). Systematic mutagenesis of deoP2 shows that the distance between the CRP site and the -10 hexamer is the crucial factor in determining whether the promoter is activated by cAMP-CRP. Based on these observations, we propose that cAMP-CRP-activated promoters can be created by correctly aligning a CRP target and a -10 hexamer. This idea has been successfully tested by converting both a CRP-independent promoter and a sequence resembling the consensus -10 hexamer to strongly cAMP-CRP-activated promoters.

引用

页码：433 / 437

页数：5

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