COMPLETE DOWN-REGULATION OF LOW-DENSITY-LIPOPROTEIN RECEPTOR ACTIVITY IN HUMAN LIVER PARENCHYMAL-CELLS BY BETA-VERY-LOW-DENSITY LIPOPROTEIN

被引：7

作者：

KAMPS, JAAM ^{[1
]}

KUIPER, J ^{[1
]}

KRUIJT, JK ^{[1
]}

VANBERKEL, TJC ^{[1
]}

机构：

[1] LEIDEN UNIV,CTR BIOPHARMACEUT SCI,DIV BIOPHARMACEUT,SYLVIUS LABS,POB 9503,2300 RA LEIDEN,NETHERLANDS

来源：

FEBS LETTERS | 1991年 / 287卷 / 1-2期

关键词：

LOW-DENSITY LIPOPROTEIN; BETA-VERY-LOW-DENSITY LIPOPROTEIN; LDL-RECEPTOR; HUMAN PARENCHYMAL LIVER CELL; RECEPTOR-REGULATION;

D O I：

10.1016/0014-5793(91)80010-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of LDL and beta-VLDL on the expression of the LDL receptor is studied in cultured human parenchymal cells. The high affinity binding of [I-125]LDL to cultured human parenchymal cells was down regulated to 37.3 +/- 2.9% and 24.0 +/- 2.6% of the control value, after preincubation with LDL or beta-VLDL for 22 h, respectively. When LDL receptor synthesis was blocked at 22 h a residual receptor activity of 29% is noticed, indicating a half-life of LDL receptors in human parenchymal cells of 12 h. It is concluded that LDL receptor expression on human liver parenchymal cells is subject to complete down-regulation by beta-VLDL, which may be held responsible for the cholesterol-rich diet induced down-regulation of LDL receptors, in vivo.

引用

页码：34 / 38

页数：5

共 29 条

[1] LIVER-TRANSPLANTATION TO PROVIDE LOW-DENSITY-LIPOPROTEIN RECEPTORS AND LOWER PLASMA-CHOLESTEROL IN A CHILD WITH HOMOZYGOUS FAMILIAL HYPERCHOLESTEROLEMIA [J].