EVIDENCE OF AN EGF/TGF-ALPHA-INDEPENDENT PATHWAY FOR ESTROGEN-REGULATED CELL-PROLIFERATION

被引：19

作者：

LEUNG, BS ^{[1
]}

STOUT, L ^{[1
]}

ZHOU, L ^{[1
]}

JI, HJ ^{[1
]}

ZHANG, QQ ^{[1
]}

LEUNG, HT ^{[1
]}

机构：

[1] UNIV MINNESOTA,DEPT ANIM PHYSIOL,MINNEAPOLIS,MN 55455

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 1991年 / 46卷 / 02期

关键词：

BREAST CANCER CELL LINE; CAMA-1; INTRON DIFFERENTIAL RNA/PCR; GENE EXPRESSION; EGF RECEPTOR;

D O I：

10.1002/jcb.240460206

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To elucidate the relationship between epidermal growth factor (EGF)/transforming growth factor (TGF-alpha) and estradiol-17-beta (E) in cell proliferation, we examined their effects on the breast cancer cell line, CAMA-1. While E was able to consistently induce cell proliferation under a variety of experimental conditions, EGF/TGF-alpha was without effect. Despite the presence of the receptor (EGFR) gene, mature EGFR protein and mRNA were not detected by radioreceptor assay, S-35 Met-labelling, and the Intron Differential RNA/PCR method under conditions in which cells remain responsive to E. Furthermore, TGF-alpha is not an autocrine factor in CAMA-1 cells. We demonstrated unequivocally that EGF/TGF-alpha interaction with EGFR is not an obligatory event in mediating estrogen-stimulated cell proliferation.

引用

页码：125 / 133

页数：9

共 34 条

[1] BLOCKADE OF THE EPIDERMAL GROWTH-FACTOR RECEPTOR INHIBITS TRANSFORMING GROWTH-FACTOR ALPHA-INDUCED BUT NOT ESTROGEN-INDUCED GROWTH OF HORMONE-DEPENDENT HUMAN-BREAST CANCER [J].