THE USE OF THE POLYMERASE CHAIN-REACTION TO IDENTIFY PORCINE ISOLATES OF TRICHINELLA

被引：40

作者：

DICK, TA

LU, MC

DEVOS, T

MA, K

机构：

来源：

JOURNAL OF PARASITOLOGY | 1992年 / 78卷 / 01期

关键词：

D O I：

10.2307/3283703

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A method was developed to identify domestic isolates of Trichinella using the polymerase chain reaction. Oligonucleotide primers, based on the repetitive DNA sequence (pPRA) from the P1 isolate of Trichinella, were used to amplify genomic DNA from 13 domestic isolates and tested against sylvatic isolates of Trichinella. Pattern differences were observed among domestic isolates, indicating divergence of this repetitive sequence. The primers were specific for domestic Trichinella as no amplification was detected for sylvatic isolates or Trichinella pseudospiralis. It was possible to identify an isolate from a single larva following digestion or in situ in muscle tissue.

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页码：145 / 148

页数：4

相关论文

共 11 条

[1] INFECTIVITY, ANTIGENICITY AND HOST RESPONSES TO ISOLATES OF THE GENUS TRICHINELLA [J].

BOLASFERNANDEZ, F ;

WAKELIN, D .

PARASITOLOGY, 1990, 100 :491-497

[2] TRICHINELLA-SPIRALIS - GENETIC-EVIDENCE FOR SYNANTHROPIC SUBSPECIES IN SYLVATIC HOSTS [J].

DAME, JB ;

MURRELL, KD ;

WORLEY, DE ;

SCHAD, GA .

EXPERIMENTAL PARASITOLOGY, 1987, 64 (02) :195-203

[3] SEQUENCE-ANALYSIS OF A 1.6KB REPETITIVE ELEMENT FROM A PORCINE ISOLATE OF TRICHINELLA-SPIRALIS [J].

DEVOS, T ;

KLASSEN, GR ;

DICK, TA .

NUCLEIC ACIDS RESEARCH, 1988, 16 (07) :3114-3114

[4]

Dick T.A., 1983, Systematics Association Special Volume Series, P351

[5] IDENTIFICATION OF 2 ISOLATES OF TRICHINELLA RECOVERED FROM HUMANS IN FRANCE [J].

DICK, TA ;

DEVOS, T ;

DUPOUYCAMET, J .

JOURNAL OF PARASITOLOGY, 1990, 76 (01) :41-44

[6] DETECTION OF REPETITIVE SEQUENCES OF TRICHINELLA-SPIRALIS BY THE POLYMERASE CHAIN-REACTION IN EXPERIMENTALLY INFECTED MICE [J].

DUPOUYCAMET, J ;

SOULE, C ;

GUILLOU, JP ;

ROUER, E ;

DESOUZA, SL ;

ANCELLE, T ;

BENAROUS, R .

PARASITOLOGY RESEARCH, 1991, 77 (02) :180-182

[7] COMPARATIVE EFFICACY OF ANTIGEN AND ANTIBODY DETECTION TESTS FOR HUMAN TRICHINELLOSIS [J].

IVANOSKA, D ;

CUPERLOVIC, K ;

GAMBLE, HR ;

MURRELL, KD .

JOURNAL OF PARASITOLOGY, 1989, 75 (01) :38-41

[8] RESTRICTION ENDONUCLEASE ANALYSIS OF REPETITIVE SEQUENCES IN THE TRICHINELLA GENOME - 3 STRAIN-SPECIFIC PATTERNS [J].

KLASSEN, GR ;

THIESSEN, JP ;

DICK, TA .

JOURNAL OF PARASITOLOGY, 1986, 72 (05) :772-775

[9] STRAIN-SPECIFIC 1.7 KILOBASE REPETITIVE DEOXYRIBONUCLEIC-ACID SEQUENCE FAMILY IN TRICHINELLA-SPIRALIS [J].

KLASSEN, GR ;

THIESSEN, JP ;

DICK, TA .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1986, 21 (03) :227-233

[10]

MURRELL KD, 1985, TRICHINELLOSIS, P301