THE EFFECTS OF TERBIUM ON THE ACCUMULATION OF CISPLATIN IN HUMAN OVARIAN-CANCER CELLS

被引:15
作者
CANADA, RG
ANDREWS, PA
MACK, KM
HAIDER, A
机构
[1] GEORGETOWN UNIV,DEPT PHARMACOL,WASHINGTON,DC 20007
[2] HOWARD UNIV,SCH LIBERAL ARTS,DEPT MATH,WASHINGTON,DC 20059
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1995年 / 1267卷 / 01期
关键词
D O I
10.1016/0167-4889(95)00019-O
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this investigation, we report a relationship between the terbium (Tb3+) binding protein and the accumulation of cisplatin in human ovarian cancer cells. The number of Tb3+ binding sites in cisplatin-resistant C13* cells is significantly greater by 79% than those in cisplatin-sensitive 2008 cells. Exposure to Tb3+ also increased the cellular accumulation of cisplatin. The accumulation of cisplatin as a function of the Tb3+ concentration in the C13* cells (0.85%/mu M Tb3+) was significantly greater than the accumulation of cisplatin in 2008 cells with respect to Tb3+ (0.46%/mu M Tb3+). The number of Tb3+ binding sites in revertant RH4 cells was similar to that in 2008 cells. The RH4 cells were less sensitive to the stimulatory effects of Tb3+ than the C13* cells. Our results show that the Tb3+ binding protein correlates with cisplatin resistance, and the receptor binding of Tb3+ increases the accumulation of cisplatin in cisplatin-resistant cells.
引用
收藏
页码:25 / 30
页数:6
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