EVIDENCE FOR THE PRESENCE OF N-UNSUBSTITUTED GLUCOSAMINE RESIDUES IN THE PEPTIDOGLYCAN OF SARCOBIUM-CYTOLYTICUM - AN INTRACELLULAR BACTERIAL PARASITE OF AMEBAS

被引:3
作者
DROZANSKI, W
MADRA, L
CHMIELEWSKI, T
SCHLECHT, S
GOLECKI, JR
机构
[1] MAX PLANCK INST IMMUNBIOL,W-7800 FREIBURG,GERMANY
[2] UNIV FREIBURG,INST BIOL 2,W-7800 FREIBURG,GERMANY
关键词
Acanthamoeba castellanii; Amoebae; Intracellular bacterial parasites; Peptidoglycan structure; Sarcobium cytolyticum;
D O I
10.1016/S0723-2020(11)80189-X
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The chemical structure of peptidoglycan of the cell wall of Sarcobium cytolyticum, an intracellular bacterial parasite of small amoebae, was investigated. The bag-shaped peptidoglycan was isolated from intact cells by a procedure involving treatments with sodium dodecyl sulfate and digestion with pronase. The final preparation was virtually free from proteins and neutral sugars. Hydrolysis of peptidoglycan with 4 N HCl at 100°C for 12 hours yielded, in addition to glucosamine, muramic acid, alanine, diaminopimelic acid, glutamic acid and glycine (molar ratiios 0.4 : 0.4 : 1.4 : 1 : 1 : 0.3), significant amounts of a disaccharide composed of glucosaminyl-β (1–4) muramic acid. Peptidoglycan peracetylated with acetic anhydride produced on acid hydrolysis only monomeric subunits represented by glucosamine, muramic acid, alanine, diaminopimelic acid, glutamic acid and glycine in a molar ratio of 1 : 1 : 1.4 : 1 : 1 : 0.3. Estimation of free NH2 groups in the native peptidoglycan by dinitrophenylation revealed that about 80% of the glucosamine residues were not substituted by acetyl groups and that only 44% of the diaminopimelic acid residues were involved in cross-linkage of peptide subunits. N-acetylation of peptidoglycan did not completely restore its sensitivity to the hydrolytic action of egg white lysozyme. © 1990, Gustav Fischer Verlag, Stuttgart · New York. All rights reserved.
引用
收藏
页码:220 / 226
页数:7
相关论文
共 38 条
[1]   ACTION OF LYSOZYME ON PEPTIDOGLYCAN WITH N-UNSUBSTITUTED GLUCOSAMINE RESIDUES - ISOLATION OF GLYCAN FRAGMENTS AND THEIR SUSCEPTIBILITY TO LYSOZYME [J].
AMANO, K ;
HAYASHI, H ;
ARAKI, Y ;
ITO, E .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1977, 76 (01) :299-307
[2]   OCCURRENCE OF NON-N-SUBSTITUTED GLUCOSAMINE RESIDUES IN LYSOZYME-RESISTANT PEPTIDOGLYCAN FROM BACILLUS CEREUS CELL WALLS [J].
ARAKI, Y ;
NAKATANI, T ;
HAYASHI, H ;
ITO, E .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1971, 42 (04) :691-&
[3]  
ARAKI Y, 1972, J BIOL CHEM, V247, P6312
[4]  
BAND RN, 1959, J GEN MICROBIOL, V20, P80
[5]   DEVELOPMENT OF LYSOZYME-RESISTANCE IN MICROCOCCUS-LYSODIEKTICUS AND ITS ASSOCIATION WITH AN INCREASED O-ACETYL CONTENT OF THE CELL WALL [J].
BRUMFITT, W ;
WARDLAW, AC ;
PARK, JT .
NATURE, 1958, 181 (4626) :1783-1784
[6]   STRUCTURE OF WALLS OF LACTOBACILLUS-ACIDOPHILUS STRAIN 63-AM GASSER [J].
COYETTE, J ;
GHUYSEN, JM .
BIOCHEMISTRY, 1970, 9 (15) :2935-&
[7]  
DEZELEE P, 1975, J BIOL CHEM, V250, P6806
[8]  
DROZANSKI W, 1984, ACTA MICROBIOL POL, V33, P195
[9]  
DROZANSKI W, 1963, ACTA MICROBIOL POL, V12, P3
[10]  
DROZANSKI W, 1979, ACTA MICROBIOL POL, V28, P123