PARTIAL CDNA CLONING AND NGF REGULATION OF A RAT 5-HT(3) RECEPTOR SUBUNIT

被引：61

作者：

ISENBERG, KE

UKHUN, IA

HOLSTAD, SG

JAFRI, S

UCHIDA, U

ZORUMSKI, CF

YANG, J

机构：

[1] ST LOUIS UNIV, COLL PHARM, DEPT PHARM PRACTICE, ST LOUIS, MO 63103 USA

[2] UNIV TEXAS, SW SCH MED, DEPT ANESTHESIOL, DALLAS, TX 75230 USA

来源：

NEUROREPORT | 1993年 / 5卷 / 02期

关键词：

NERVE GROWTH FACTOR; 5-HT3; RECEPTOR; PC12; CELLS; STEADY-STATE MESSENGER RNA LEVELS; POSTTRANSCRIPTIONAL EFFECTS;

D O I：

10.1097/00001756-199311180-00006

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

NERVE growth factor (NGF) stimulates 5-HT, receptor expression although the mechanism(s) responsible for this effect are unknown. We report the nucleotide and deduced amino acid sequences of a nearly complete rat 5-HT, receptor subunit and use the sequence to develop a reverse transcription-polymerase chain reaction (RT-PCR) assay that measures 5-HT, mRNA. Exposure of PC12 cells to NGF results in increasing steady-state levels of 5-HT, mRNA. The four-fold increase in mRNA correlates with and is sufficient to account for increases in receptor measured by agonist induction of whole cell currents.

引用

页码：121 / 124

页数：4

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