STEREOSELECTIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF THE ENANTIOMERS OF KETAMINE AND NORKETAMINE IN PLASMA

被引：57

作者：

GEISSLINGER, G ^{[1
]}

MENZELSOGLOWEK, S ^{[1
]}

KAMP, HD ^{[1
]}

BRUNE, K ^{[1
]}

机构：

[1] UNIV ERLANGEN NURNBERG,DEPT ANESTHESIOL,W-8520 ERLANGEN,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 568卷 / 01期

关键词：

D O I：

10.1016/0378-4347(91)80350-L

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An enantioselective high-performance liquid chromatographic assay for the quantitation of the enantiomers of ketamine and its major metabolite norketamine in human plasma is described (assay I). The procedure involved extraction of the compounds from alkalized plasma into cyclohexane. Stereoselective separation was achieved with a prepacked alpha-1-acid glycoprotein column without any derivatization procedure. A second assay using a conventional reversed-phase column to determine total (racemic) ketamine and norketamine is also described. Because of interfering plasma peaks (assay II) the cyclohexane solution was reextracted into 1 M hydrochloric acid. The detection wavelength was 215 nm for all substances. The limit of quantification of the method was ca. 40 ng/ml in plasma. The assays were sensitive and reproducible. The method was demonstrated to be sensitive for stereoselective pharmacokinetic studies of ketamine after clinical doses.

引用

页码：165 / 176

页数：12

共 29 条

[1] DERIVATIZATION OF CHIRAL AMINES WITH (S,S)-N-TRIFLUOROACETYLPROLINE ANHYDRIDE FOR GC ESTIMATION OF ENANTIOMERIC COMPOSITION [J].

ADAMS, JD ;

WOOLF, TF ;

TREVOR, AJ ;

WILLIAMS, LR ;

CASTAGNOLI, N .

JOURNAL OF PHARMACEUTICAL SCIENCES, 1982, 71 (06) :658-661

[2] STUDIES ON THE BIOTRANSFORMATION OF KETAMINE .1. IDENTIFICATION OF METABOLITES PRODUCED INVITRO FROM RAT-LIVER MICROSOMAL PREPARATIONS [J].

ADAMS, JD ;

BAILLIE, TA ;

TREVOR, AJ ;

CASTAGNOLI, N .

BIOMEDICAL MASS SPECTROMETRY, 1981, 8 (11) :527-538

[3] STEREOCHEMISTRY - A SOURCE OF PROBLEMS IN MEDICINAL CHEMISTRY [J].

ARIENS, EJ .

MEDICINAL RESEARCH REVIEWS, 1986, 6 (04) :451-466

[4] DETERMINATION OF THE ENANTIOMERS OF KETOPROFEN IN BLOOD-PLASMA BY ION-PAIR EXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF LEUCINAMIDE DERIVATIVES [J].

BJORKMAN, S .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1987, 414 (02) :465-471

[5]

BLASCHKE G, 1988, ANAL TASCHENBUCH, V7, P123

[6] GAS-CHROMATOGRAPHIC ASSAY FOR KETAMINE IN HUMAN PLASMA [J].

CHANG, T ;

GLAZKO, AJ .

ANESTHESIOLOGY, 1972, 36 (04) :401-&

[7]

CHANG T, 1974, International Anesthesiology Clinics, V12, P157, DOI 10.1097/00004311-197412020-00018

[8]

CHANG T, 1965, FED PROC, V24, P268

[9] DISTRIBUTION IN BRAIN AND METABOLISM OF KETAMINE IN RAT AFTER INTRAVENOUS ADMINISTRATION [J].

COHEN, ML ;

CHAN, SL ;

WAY, WL ;

TREVOR, AJ .

ANESTHESIOLOGY, 1973, 39 (04) :370-376

[10]

CORSSEN G, 1966, ANESTH ANAL CURR RES, V45, P29

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