EVALUATION OF CELL ATTACHMENT MATRIX (CAM) COATED PLATES FOR PRIMARY CULTURE OF HUMAN TUMOR-BIOPSIES

被引：10

作者：

PRICE, P

BUSH, C

PARKINS, CS

MCMILLAN, TJ

ROBINSON, M

STEEL, GG

机构：

[1] INST CANC RES,RADIOTHERAPY RES UNIT,SUTTON SM2 5PX,SURREY,ENGLAND

[2] ROYAL MARSDEN HOSP,DEPT RADIOTHERAPY,LONDON,ENGLAND

来源：

RADIOTHERAPY AND ONCOLOGY | 1991年 / 21卷 / 04期

关键词：

PRIMARY TUMOR; RADIOSENSITIVITY TESTING; CELL ATTACHMENT MATRIX PLATES; CELL CULTURE;

D O I：

10.1016/0167-8140(91)90055-L

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Baker et al. [Cancer Res. 46: 1263-1274, 1986] developed an adhesive tumour cell culture system (ATCCS) using a culture surface coated with a cell attachment matrix (CAM). The ability of CAM-coated plates to support the growth of cells from human tumour biopsies has been evaluated. Successful growth was obtained in 9/22 samples (41%), but fibroblasts, rather than tumour cells, grew in the majority. Comparison of CAM with other surfaces showed that CAM was no better for establishing tumour cell growth than the presence of feeder cells or an alternative attachment factor vitronectin.

引用

页码：282 / 285

页数：4

共 12 条

[1] COMPARISON BETWEEN CLINICAL-RESPONSE AND INVITRO DRUG SENSITIVITY OF PRIMARY HUMAN-TUMORS IN THE ADHESIVE TUMOR-CELL CULTURE SYSTEM [J].