DETERMINATION BY SYSTEMATIC DELETION OF THE AMINO-ACIDS ESSENTIAL FOR CATALYSIS BY RICIN-A CHAIN

被引：46

作者：

MORRIS, KN

WOOL, IG

机构：

[1] Biochemistry/Molecular Biology Dept., University of Chicago, Chicago

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 11期

关键词：

D O I：

10.1073/pnas.89.11.4869

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The A chain of ricin is a RNA N-glycosidase that inactivates ribosomes by depurination of a single adenosine in 28S rRNA. Of the 267 amino acids in the protein, 222 (83 %) could be deleted from one or another of 74 mutants without loss of the capacity of the protein to recognize a single nucleotide from among the 7000 in ribosomes or to catalyze hydrolysis.

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页码：4869 / 4873

页数：5

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