FEMALE AND MALE GREEN MONKEY LIVER ESTROGEN-RECEPTOR

Adult female and male green monkey liver cytosol contains tritiated 17β-estradiol ([3H]E2) binding sites. The binding of [3H]E2 to these sites is reduced substantially by diethylstilbestrol. Unlike adult male rate liver cytosol, neither male nor female green monkey liver cytosol contains moderate affinity high capacity [3H]E2 binding sites. The green monkey liver cytosol [3H]E2 sites are precipitated by ammonium sulfate at 30% of saturation. The properties of the redissolved ammonium sulfate precipitated female and male [3H]E2 binding sites are similar: they have a high [3H]E2 affinity, a low [3H]E2 capacity, are estrogen specific, and are sensitive to sulfhydryl inactivation and protease digestion. Although the female and male sites are similar, there appears to be a 2- to 3-fold sex difference in the affinity (female Kd, 0.8 to 1.3 × 10-10 M; male Kd, 0.4 × 10-10M) and capacity (female, 2.3 to 3.7 fmoles/mg of liver; male, 1.2 fmoles/mg of liver)of the [3H]E2 binding sites. Accordingly, adult female and male green monkey liver cytosol contains putative estradiol receptors. © 1979.