A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE QUANTITATION OF FLECAINIDE IN PLASMA

被引：6

作者：

BROLY, F

MARECAUX, P

HOUDRET, N

LHERMITTE, M

HOUSSIN, R

机构：

[1] HOP CALMETTE,BIOCHIM LAB,RUE PR J LECLERCQ,F-59037 LILLE,FRANCE

[2] FAC PHARM LILLE,CHIM THERAPEUT LAB,F-59045 LILLE,FRANCE

[3] FAC PHARM LILLE,TOXICOL LAB,F-59045 LILLE,FRANCE

来源：

BIOMEDICAL CHROMATOGRAPHY | 1991年 / 5卷 / 06期

关键词：

D O I：

10.1002/bmc.1130050604

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A routine high performance liquid chromatographic method for the rapid determination of flecainide (Flecaine(R)), using a novel internal standard, N-methylflecainide, has been developed. After deproteinization of spiked samples, flecainide was totally recovered at neutral pH. Flecainide and the internal standard were separated on a reversed phase XL 3-mu-m ODS column using 10 mM phosphate buffer, pH 3.0: acetonitrile (70:30) as mobile phase, in less than 10 min. With spectrofluorometric detection, the limit of quantitation for flecainide was 10 ng/mL. Intra- and inter-assay precision variations were 0.24% and 1.4%.

引用

收藏

页码：248 / 250

页数：3

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