CONSTRUCTION OF A SHUTTLE VECTOR FOR USE BETWEEN PASTEURELLA-MULTOCIDA AND ESCHERICHIA-COLI

被引：11

作者：

BILLS, MM ^{[1
]}

MEDD, JM ^{[1
]}

CHAPPEL, RJ ^{[1
]}

ADLER, B ^{[1
]}

机构：

[1] MONASH UNIV,DEPT MICROBIOL,CLAYTON,VIC 3168,AUSTRALIA

来源：

PLASMID | 1993年 / 30卷 / 03期

关键词：

D O I：

10.1006/plas.1993.1058

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The isolation of a small plasmid from Pasteurella multocida has enabled the construction of a shuttle vector for use between P. multocida and Escherichia coli. The vector pBAC64 contains the origin of replication from P. multocida, an antibiotic resistance gene which functions in P. multocida, and the E. coli vector pUC18. The presence of the pUC18 multiple cloning site together with the lacZ' gene provides a screening method and allows cloning and manipulation in E. coli as well as cloning in P. multocida. © 1993 Academic Press, Inc.

引用

页码：268 / 273

页数：6

共 15 条

[1]

ADLAM C, 1989, PASTEURELLA PASTUREL

[2]

Ausubel FM., 1995, MOL REPROD DEV, V3rd edn, DOI DOI 10.1002/MRD.1080010210

[3] PLASMIDS RELATED TO THE BROAD HOST RANGE VECTOR, PRK290, USEFUL FOR GENE CLONING AND FOR MONITORING GENE-EXPRESSION [J].