MOLECULAR-CLONING OF RAT KYNURENINE AMINOTRANSFERASE - IDENTITY WITH GLUTAMINE TRANSAMINASE-K

被引：52

作者：

MOSCA, M

COZZI, L

BRETON, J

SPECIALE, C

OKUNO, E

SCHWARCZ, R

BENATTI, L

机构：

[1] BU THERAPEUT PRECLIN RES,PHARM RES CTR,I-20014 NERVIANO,ITALY

[2] BIOSCI CTR,NERVIANO,ITALY

[3] UNIV MARYLAND,SCH MED,MARYLAND PSYCHIAT RES CTR,BALTIMORE,MD

来源：

FEBS LETTERS | 1994年 / 353卷 / 01期

关键词：

KYNURENINE AMINOTRANSFERASE; GLUTAMINE TRANSAMINASE K; CYSTEINE CONJUGATE P-LYASE; EXCITATORY AMINO ACID; NEUROPROTECTION; KYNURENIC ACID;

D O I：

10.1016/0014-5793(94)01003-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The enzyme kynurenine aminotransferase (KAT) catalyses the conversion of L-kynurenine to kynurenic acid. A combination of polymerase chain reaction techniques and hybridization screening was used to isolate a cDNA clone encompassing the entire coding region of KAT from rat kidney. Identification of the cDNA as coding for KAT was based both on the comparison of amino acid sequences obtained from purified rat KAT and on the expression of KAT activity in COS-1 cells transfected with the cDNA. RNA blot analysis indicated that KAT mRNA is widely expressed in rat tissues. Cultured cells transfected with the cDNA for KAT also showed glutamine transaminase K activity. Based mainly on sequence data, these results demonstrate that rat kidney KAT is identical with glutamine transaminase K.

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页码：21 / 24

页数：4

相关论文

共 17 条

[1] GLUTAMINE TRANSAMINASE-K AND CYSTEINE-S-CONJUGATE BETA-LYASE ACTIVITY STAINS [J].

ABRAHAM, DG ;

COOPER, AJL .

ANALYTICAL BIOCHEMISTRY, 1991, 197 (02) :421-427

[2] NUCLEOTIDE-SEQUENCE OF CDNA CODING FOR SAPORIN-6, A TYPE-1 RIBOSOME-INACTIVATING PROTEIN FROM SAPONARIA-OFFICINALIS [J].

BENATTI, L ;

SACCARDO, MB ;

DANI, M ;

NITTI, G ;

SASSANO, M ;

LORENZETTI, R ;

LAPPI, DA ;

SORIA, M .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 183 (02) :465-470

[3]

COOPER AJL, 1985, METHOD ENZYMOL, V113, P344

[4] THE SIGNAL PEPTIDE OF HUMAN PREPROENDOTHELIN-1 [J].

FABBRINI, MS ;

VALSASINA, B ;

NITTI, G ;

BENATTI, L ;

VITALE, A .

FEBS LETTERS, 1991, 286 (1-2) :91-94

[5] KYNURENIC ACID BLOCKS NEUROTOXICITY AND SEIZURES INDUCED IN RATS BY THE RELATED BRAIN METABOLITE QUINOLINIC ACID [J].

FOSTER, AC ;

VEZZANI, A ;

FRENCH, ED ;

SCHWARCZ, R .

NEUROSCIENCE LETTERS, 1984, 48 (03) :273-278

[6] KYNURENATE INHIBITION OF CELL EXCITATION DECREASES STROKE SIZE AND DEFICITS [J].

GERMANO, IM ;

PITTS, LH ;

MELDRUM, BS ;

BARTKOWSKI, HM ;

SIMON, RP .

ANNALS OF NEUROLOGY, 1987, 22 (06) :730-734

[7] PURIFICATION AND CHARACTERIZATION OF KYNURENINE-2-OXOGLUTARATE AMINOTRANSFERASE FROM LIVER, BRAIN AND SMALL-INTESTINE OF RATS [J].

NOGUCHI, T ;

MINATOGAWA, Y ;

OKUNO, E ;

NAKATANI, M ;

MORIMOTO, M ;

KIDO, R .

BIOCHEMICAL JOURNAL, 1975, 151 (02) :399-406

[8] MEASUREMENT OF RAT-BRAIN KYNURENINE AMINOTRANSFERASE AT PHYSIOLOGICAL KYNURENINE CONCENTRATIONS [J].

OKUNO, E ;

SCHMIDT, W ;

PARKS, DA ;

NAKAMURA, M ;

SCHWARCZ, R .

JOURNAL OF NEUROCHEMISTRY, 1991, 57 (02) :533-540

[9] CRYSTALLIZATION AND CHARACTERIZATION OF HUMAN-LIVER KYNURENINE-GLYOXYLATE AMINOTRANSFERASE - IDENTITY WITH ALANINE-GLYOXYLATE AMINOTRANSFERASE [J].

OKUNO, E ;

MINATOGAWA, Y ;

NAKAMURA, M ;

KAMODA, N ;

NAKANISHI, J ;

MAKINO, M ;

KIDO, R .

BIOCHEMICAL JOURNAL, 1980, 189 (03) :581-590

[10] PURIFICATION AND CHARACTERIZATION OF KYNURENINE-PYRUVATE AMINOTRANSFERASE FROM RAT-KIDNEY AND BRAIN [J].

OKUNO, E ;

DU, F ;

ISHIKAWA, T ;

TSUJIMOTO, M ;

NAKAMURA, M ;

SCHWARCZ, R ;

KIDO, R .

BRAIN RESEARCH, 1990, 534 (1-2) :37-44