STREPTOCOCCAL PYROGENIC EXOTOXIN-A AND STREPTOLYSIN-O ENHANCE POLYMORPHONUCLEAR LEUKOCYTE BINDING TO GELATIN MATRICES

被引:14
作者
BRYANT, AE
KEHOE, MA
STEVENS, DL
机构
[1] VET ADM MED CTR, INFECT DIS SECT T109, 500 W FT ST, BOISE, ID 83702 USA
[2] UNIV NEWCASTLE UPON TYNE, DEPT MICROBIOL, NEWCASTLE TYNE NE1 7RU, TYNE & WEAR, ENGLAND
[3] UNIV WASHINGTON, SCH MED, SEATTLE, WA 98195 USA
基金
英国医学研究理事会;
关键词
D O I
10.1093/infdis/166.1.165
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Autopsy data from cases of streptococcal toxic shock demonstrate accumulation of polymorphonuclear leukocytes (PMNL) within lung and soft tissue microvasculature. Because of the increased prevalence of streptococcal pyrogenic exotoxin A (SPEA)-producing strains associated with streptococcal toxic shock syndrome, experiments were done to determine whether SPEA or streptolysin O (SLO, a thiol-activated cytolysin produced by all group A streptococci) could stimulate PMNL-dependent adherence mechanisms in vitro. SPEA (0.01-10-mu-g/5.5 x 10(6) PMNL) only modestly enhanced PMNL adherence over the entire range of concentrations tested. In contrast, SLO-induced PMNL binding was highly dose dependent (maximal binding, 55.1 +/- 1.6% at 0.5 hemolytic units/5.5 x 10(6) PMNL) and was mediated by CD11/CD18 adherence glycoprotein.
引用
收藏
页码:165 / 169
页数:5
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