EVALUATION OF REVERSED-PHASE COLUMNS FOR THE ANALYSIS OF VERY BASIC COMPOUNDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - APPLICATION TO THE DETERMINATION OF THE TOBACCO ALKALOIDS

被引：72

作者：

MCCALLEY, DV

机构：

[1] Department of Chemical and Physical Sciences, University of the West of England, Bristol, BS16 1QY, Frenchay

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 636卷 / 02期

关键词：

D O I：

10.1016/0021-9673(93)80234-Y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid separation of the major tobacco alkaloids with high efficiency and acceptable peak tailing is demonstrated on a RP-HPLC column containing a high-purity silica substrate, without use of silanol masking agents in the eluent. A test procedure based on that of Engelhardt and co-workers, with pyridine as an additional probe compound, was used to aid the selection of a suitable stationary phase from a number of the best commercially available columns for the separation of strongly basic compounds.

引用

页码：213 / 220

页数：8

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