ALTERATIONS OF THE ETHYLENE PATHWAY IN GERMINATING THERMOINHIBITED CHICKPEA SEEDS CAUSED BY THE INHIBITION OF POLYAMINE BIOSYNTHESIS

被引：16

作者：

GALLARDO, M

DERUEDA, PM

MATILLA, AJ

SANCHEZCALLE, IM

机构：

[1] UNIV GRANADA, FAC CIENCIAS, DEPT BIOL VEGETAL, E-18001 GRANADA, SPAIN

[2] UNIV GRANADA, FAC CIENCIAS, INST BIOTECHNOL, E-18001 GRANADA, SPAIN

来源：

PLANT SCIENCE | 1995年 / 104卷 / 02期

关键词：

ETHYLENE; POLYAMINES; CICER ARIETINUM; GERMINATION;

D O I：

10.1016/0168-9452(94)04019-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The alterations in the ethylene biosynthetic pathway provoked by cyclohexylamine (CHA) and/or methylglyoxalbis(guanylhydrazone) (MGBG), inhibitors of the polyamine synthesis, have been studied in thermoinhibited seeds (delayed germination by supraoptimal temperatures) of the chick-pea (Cicer arietinum). CHA and/or MGBG stimulated ethylene synthesis both at 25 degrees C (control) and at 30 degrees C (temperature at which thermoinhibition is induced, and reversed by both inhibitors). Moreover, both inhibitors caused: (1) an increase in ACC-synthase (EC 4.4.1.14) and free ACC; (2) a stimulation of in vivo ACC-oxidase; and (3) a stimulation of (malonyl)-1-aminocyclopropane-1-carboxylic acid synthesis at 25 degrees C (since the supraoptimal temperatures had no effect). Compared with the control, both supraoptimal temperatures lead to a decrease the AdoMet-decarboxylase (AdoMetDC) (EC 4.1.1.50) activity. This was not affected by CHA. However, MGBG induced an increase of this activity at 25 degrees C and at 30 degrees C, which diminished strongly up to 35 degrees C. The fact that the inhibition of spermidine and spermine synthesis by CHA and/or MGBG provoked an induction of the enzyme of ethylene biosynthesis and a concomitant increase in ethylene production and a reversion of thermoinhibition in chick-pea seeds suggests that the ethylene and polyamine pathways compete during germination, placing polyamines in the possible role of an endogenous germination regulator possibly by modulating ethylene biosynthesis.

引用

页码：169 / 175

页数：7

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