EFFECTS OF CD++ ON SHORT-CIRCUIT CURRENT ACROSS EPITHELIAL MEMBRANES .2. STUDIES WITH THE ISOLATED FROG-SKIN EPITHELIUM, URINARY-BLADDER, AND LARGE-INTESTINE

Cadmium ion (Cd++) was found not to inhibit active sodium transport across the isolated frog skin when added in 10-3m concentration to the basal-lateral surface. The same Cd++ concentration similarly had no effect on Na+ transport across the isolated epithelial cell layer from the frog skin, although this dose of Cd++ did inhibit Na+ transport across the frog urinary bladder and large intestine. When 10-3m Cd++ was added to the apical surface of the isolated frog skin or to the isolated epithelial cells from the frog skin, sodium transport was reversibly stimulated, in contrast to the irreversible inhibition noted above. If equimolar cysteine was added with Cd++ to the apical surface of the skin, however, active Na+ transport was irreversibly inhibited. In conjunction with the inhibition produced by equimolar Cd++ and cysteine, isotopic Cd++ permeation into the tissue was three times higher when added with cysteine than in the absence of cysteine. Thus, the effects of Cd++ on epithelial Na+ transport is variable according to the epithelium studied and the presence of potential carrier molecules. © 1979 Springer-Verlag New York Inc.