A NEW ASSAY FOR TRANSGLUTAMINASE

被引：8

作者：

SEIVING, B

STENBERG, P

NILSSON, B

机构：

[1] MALMO GEN HOSP,S-21401 MALMO,SWEDEN

[2] CELLALYS,BJARRED,SWEDEN

来源：

SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION | 1991年 / 51卷 / 02期

关键词：

BIOTIN; CASEIN; FACTOR-XIII;

D O I：

10.3109/00365519109091096

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

A new solid-phase micro assay for transglutaminase has been developed. Casein bound to microtitre plates and biotin-labelled casein were used as substrates in a transglutaminase-dependent cross-linking reaction. The resulting immobilized biotin was visualized by addition of avidin-labelled alkaline phosphatase followed by p-nitrophenyl phosphate. The colour development was monitored at 405 nm. Tissue transglutaminase was used as test enzyme. The method was also applied to normal and Factor XIII-deficient plasma.

引用

页码：119 / 124

页数：6

共 12 条

[1] CALCIUM-DEPENDENT UNMASKING OF ACTIVE-CENTER CYSTEINE DURING ACTIVATION OF FIBRIN STABILIZING FACTOR
CURTIS, CG
BROWN, KL
CREDO, RB
DOMANIK, RA
GRAY, A
STENBERG, P
LORAND, L
[J]. BIOCHEMISTRY, 1974, 13 (18) : 3774 - 3780
[2] TRANSGLUTAMINASE AND RECEPTOR-MEDIATED ENDOCYTOSIS IN MACROPHAGES AND CULTURED FIBROBLASTS
DAVIES, PJA
MURTAUGH, MP
[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY, 1984, 58 (1-2) : 69 - 77
[3] QUANTITATION OF TISSUE TRANSGLUTAMINASE BY A SANDWICH ELISA SYSTEM
FESUS, L
ARATO, G
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1986, 94 (1-2) : 131 - 136
[4] Folk J E, 1977, Adv Protein Chem, V31, P1, DOI 10.1016/S0065-3233(08)60217-X
[5] EVIDENCE FOR PARTICIPATION OF TRANSGLUTAMINASE IN RECEPTOR-MEDIATED ENDOCYTOSIS
LEVITZKI, A
WILLINGHAM, M
PASTAN, I
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (05): : 2706 - 2710
[6] FIBRINOLIGASE - FIBRIN-STABILIZING FACTOR SYSTEM OF BLOOD-PLASMA
LORAND, L
[J]. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1972, 202 (DEC8) : 6 - 30
[7] TRANSGLUTAMINASES
LORAND, L
CONRAD, SM
[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY, 1984, 58 (1-2) : 9 - 35
[8] DISSOCIATION OF SUBUNIT STRUCTURE OF FIBRIN STABILIZING FACTOR DURING ACTIVATION OF ZYMOGEN
LORAND, L
GRAY, AJ
BROWN, K
CREDO, RB
CURTIS, CG
DOMANIK, RA
STENBERG, P
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1974, 56 (04) : 914 - 922
[9] FILTER PAPER ASSAY FOR TRANSAMIDATING ENZYMES USING RADIOACTIVE AMINE SUBSTRATES
LORAND, L
COOPERST.L
CAMPBELL.LK
[J]. ANALYTICAL BIOCHEMISTRY, 1972, 50 (02) : 623 - &
[10] INDUCTION OF TISSUE TRANSGLUTAMINASE IN HUMAN PERIPHERAL-BLOOD MONOCYTES
MURTAUGH, MP
AREND, WP
DAVIES, PJA
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1984, 159 (01) : 114 - 125

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