KINETIC DETERMINATION OF TALIN-ACTIN BINDING

被引:38
作者
GOLDMANN, WH
ISENBERG, G
机构
关键词
D O I
10.1016/0006-291X(91)90167-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Smooth muscle talin prepared from chicken gizzard binds to skeletal muscle actin in vitro. The stoichiometry of 1:3 for talin : fluorescent labelled G-actin was confirmed by steady state titration and viscosity measurements under non-polymerizing conditions. The binding constant (Kd) of talin and G-actin was determined by continuous fluorescence titration and gave a value of approx 0.3μM. The association rate constant of talin and fluorescent labelled G-actin of approx 7×106 M-1 × s-1 was ascertained by the stopped flow method; the dissociation rate constant was calculated at approx 2-3 s-1. © 1991.
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页码:718 / 723
页数:6
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