CITRIC-ACID PRODUCTION FROM GLUCOSE .1. GROWTH AND EXCRETION KINETICS IN A STIRRED FERMENTOR

被引:51
作者
BRIFFAUD, J
ENGASSER, M
机构
[1] CNRS,ECOLE NATL SUPER IND CHIM,SCI GENIE LAB,1 RUE GRANDVILLE,F-54042 NANCY,FRANCE
[2] INST FRANCAIS PETR,BIOCHEM LAB,PARIS,FRANCE
关键词
D O I
10.1002/bit.260211113
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The growth and citric acid production kinetics of Saccharomycopsis lipolytica on glucose are investigated in an aerated stirred fermentor. Cellular growth first proceeds exponentially until exhaustion of ammonia in the fermentation medium. Cells then continue to grow at a reduced rate with a concomitant decrease in intracellular nitrogen content. Citric and isocitric acid production starts at the end of the growth phase. During about 80 hr excretion proceeds at a constant rate of 0.7 g/liter/hr for citric acid and 0.1 g/liter/hr for isocitric acid. The final citric and isocitric acid concentrations are 95 and 10g/liter, respectively. During acid excretion cellular respiration accounts for 60 and 35% of consumed oxygen and glucose. Both acid and CO2 production rates follow a Michaelis–Menten‐type dependence on oxygen concentration with Michaelis–Menten constants of 0.9 and 0.15 mg/liter for acid and CO2 productions, respectively. Copyright © 1979 John Wiley & Sons, Inc.
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页码:2083 / 2092
页数:10
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