PURIFICATION AND CHARACTERIZATION OF PREGNANCY ASSOCIATED PLASMA PROTEIN-A (PAPP-A)

被引：81

作者：

BISCHOF, P

机构：

[1] UNIV ABERDEEN, DEPT OBSTET & GYNAECOL, ABERDEEN AB9 1FX, SCOTLAND

[2] UNIV ABERDEEN, DEPT BIOCHEM, ABERDEEN AB9 1FX, SCOTLAND

来源：

ARCHIVES OF GYNECOLOGY | 1979年 / 227卷 / 04期

关键词：

Complement inhibition; Fibrinolytic inhibition; Placental protein; Pregnancy Associated Plasma Protein A (PAPP-A);

D O I：

10.1007/BF02109920

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Pregnancy Associated Plasma Protein A (PAPP-A) has been isolated from late pregnancy plasma using ammonium sulphate precipitation, ion exchange chromatography, affinity chromatography on Concanavalin-A, gel filtration and negative affinity chromatography. It was found that PAPP-A is an α2-glycoprotein of 750-820 000 MW, probably a dimer with each monomer being composed of 2 polypeptide chains of 218 000 MW. The amino acid composition as well as other physicochemical characteristics are similar to human α2-macroglobulin. PAPP-A exhibits in vitro an inhibition of the activity of the complement system, of the caseinolytic activity of plasmin and possibly of the urokinase activation of plasminogen. The hypothesis that PAPP-A plays a role in the regulation of fibrinolysis during pregnancy is put forward. © 1979 J. F. Bergmann Verlag.

引用

页码：315 / 326

页数：12

共 23 条

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