GLUTAMATE INDUCES THE GROWTH-FACTORS NGF, BFGF, THE RECEPTOR FGF-R1 AND C-FOS MESSENGER-RNA EXPRESSION IN RAT ASTROCYTE CULTURE

被引：74

作者：

PECHAN, PA

CHOWDHURY, K

GERDES, W

SEIFERT, W

机构：

[1] MAX PLANCK INST BIOPHYS CHEM,DEPT NEUROBIOL,MOLEC NEUROBIOL LAB,POB 2841,W-3400 GOTTINGEN,GERMANY

[2] MAX PLANCK INST BIOPHYS CHEM,DEPT MOLEC CELL BIOL,W-3400 GOTTINGEN,GERMANY

来源：

NEUROSCIENCE LETTERS | 1993年 / 153卷 / 01期

关键词：

GLUTAMATE; BFGF; NGF; C-FOS; FGF RECEPTOR; GENE EXPRESSION; RAT ASTROCYTE; PRIMARY CULTURE;

D O I：

10.1016/0304-3940(93)90089-4

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The effect of glutamate on primary cultures of rat cortical astrocytes was studied using Northern blot hybridization. Incubation with glutamate (100 muM, 15 min) induced nerve growth factor (NGF), basic fibroblast growth factor (bFGF), FGF receptor (FGF-R1) and proto-oncogene c-fos gene expression in a time dependent manner. Maximal induction of NGF, bFGF and FGF-R1 mRNA was reached after 4 h of incubation (7.2-fold induction of NGF, 3-fold increase in bFGF and 3.6-fold induction of FGF-R1 mRNA). The induction kinetics of NGF, bFGF and FGF-R1 mRNA are similar. The rapid (1 h) 77-fold induction of the c-fos transcript preceeds the induction of the other genes tested.

引用

页码：111 / 114

页数：4

共 26 条

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