CLONING AND SEQUENCE-ANALYSIS OF A CDNA CODING FOR THE MURINE DNA-LIGASE-I ENZYME

被引：16

作者：

SAVINI, E ^{[1
]}

BIAMONTI, G ^{[1
]}

CIARROCCHI, G ^{[1
]}

MONTECUCCO, A ^{[1
]}

机构：

[1] CNR,IST GENET BIOCHIM & EVOLUZ,I-27100 PAVIA,ITALY

来源：

GENE | 1994年 / 144卷 / 02期

关键词：

RECOMBINANT DNA; MOUSE CDNA LIBRARY; NUCLEOTIDE SEQUENCE; GENE EXPRESSION; NIH-3T3; CELLS; PROTEIN STRUCTURE;

D O I：

10.1016/0378-1119(94)90386-7

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A complementary DNA (2961 bp) containing the complete coding sequence for murine DNA ligase I was isolated from a mouse fibroblast cDNA library using a cDNA encoding the human protein as a probe. An open reading frame of 2748 bp, encoding a protein of 916 amino acids (aa), was identified. Northern blot analysis of total RNA extracted from mouse fibroblasts showed a single band with a mobility corresponding to a size of 3.2 kb whose level increases upon serum stimulation of quiescent mouse NIH-3T3 cells. Alignment of the murine and human deduced aa sequences showed an overall 83% identity, that rises to 91% if only the sequence on the C-terminal portion of the protein containing the active site is considered.

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页码：253 / 257

页数：5

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