FILTER SPOT-ELISA FOR THE ENUMERATION OF INTERFERON-ALPHA ANTIBODY-SECRETING CELLS

被引：4

作者：

PRUMMER, O

CEDERBLAD, B

ALM, G

DREES, N

PORZSOLT, F

机构：

[1] HOFFMAN LA ROCHE INC,W-7889 GRENZACH,GERMANY

[2] UNIV UPPSALA,INTERFERON LAB,S-75105 UPPSALA,SWEDEN

[3] SWEDISH UNIV AGR SCI,DEPT VET IMMUNOL,S-75007 UPPSALA,SWEDEN

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 130卷 / 02期

关键词：

Antibody-secreting cell; ELISA; Hybridoma; Interferon antibody; Nitrocellulose; spot-;

D O I：

10.1016/0022-1759(90)90047-Y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A filter spot-ELISA was developed for the enumeration of interferon-α (IFN-α) antibody secretion by murine and human cells. Various human IFN-α subtypes were coated onto nitrocellulose membranes by means of broad specificity bovine antibodies. Membranes were blocked with milk proteins, and antibodies released by individual celld during a 3 h culture period were visualized as distinct spots using peroxidase-conjugated, species-specific anti-immunoglobulin antibodies and diaminobenzidine/hydrogen peroxide substrate solution. The spot-ELISA is rapid, easy to perform, and highly sensitive. Possible applications include frequency estimates of IFN-α antibody-secreting cells in blood, tissues and hybridoma cultures as well as the evaluation of specificity and immunoglobulin class of the respective antibodies. © 1990.

引用

页码：187 / 193

页数：7

共 18 条

[1] PROTEIN BLOTTING
BEISIEGEL, U
[J]. ELECTROPHORESIS, 1986, 7 (01) : 1 - 18
[2] A NOVEL 2 COLOR ELISPOT ASSAY .1. SIMULTANEOUS DETECTION OF DISTINCT TYPES OF ANTIBODY-SECRETING CELLS
CZERKINSKY, C
MOLDOVEANU, Z
MESTECKY, J
NILSSON, LA
OUCHTERLONY, O
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1988, 115 (01) : 31 - 37
[3] REVERSE ENZYME-LINKED IMMUNOSPOT ASSAY (RELISPOT) FOR THE DETECTION OF CELLS SECRETING IMMUNOREACTIVE SUBSTANCES
CZERKINSKY, CC
TARKOWSKI, A
NILSSON, LA
OUCHTERLONY, O
NYGREN, H
GRETZER, C
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1984, 72 (02) : 489 - 496
[4] A SOLID-PHASE ENZYME-LINKED IMMUNOSPOT (ELISPOT) ASSAY FOR ENUMERATION OF SPECIFIC ANTIBODY-SECRETING CELLS
CZERKINSKY, CC
NILSSON, LA
NYGREN, H
OUCHTERLONY, O
TARKOWSKI, A
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1983, 65 (1-2) : 109 - 121
[5] THE DETECTION OF ANTIBODIES TO RECOMBINANT INTERFERON ALFA-2A IN HUMAN-SERUM
HENNES, U
JUCKER, W
FISCHER, EA
KRUMMENACHER, T
PALLERONI, AV
TROWN, PW
LINDERCICCOLUNGHI, S
RAINISIO, M
[J]. JOURNAL OF BIOLOGICAL STANDARDIZATION, 1987, 15 (03): : 231 - 244
[6] ITRI LM, 1987, CANCER, V59, P668, DOI 10.1002/1097-0142(19870201)59:3+<668::AID-CNCR2820591317>3.0.CO
[7] 2-J
[8] PLAQUE FORMATION IN AGAR BY SINGLE ANTIBODY-PRODUCING CELLS
JERNE, NK
NORDIN, AA
[J]. SCIENCE, 1963, 140 (356) : 405 - &
[9] Johnson D. A., 1984, Gene Analysis Techniques, V1, P3, DOI 10.1016/0735-0651(84)90049-9
[10] CONTINUOUS CULTURES OF FUSED CELLS SECRETING ANTIBODY OF PREDEFINED SPECIFICITY
KOHLER, G
MILSTEIN, C
[J]. NATURE, 1975, 256 (5517) : 495 - 497

← 1 2 →