PROCEDURE FOR THE CLONING AND IDENTIFICATION OF Y-SPECIFIC MIDDLE REPETITIVE SEQUENCES IN DROSOPHILA-HYDEI

Clones of hybrid plasmids containing moderately and highly repeated sequences of Drosophila hydei exhibit positive autoradiographic signals if hybridized to labeled whole genome DNA. Such clones were screened with labeled male ( X Y) and female ( X X) DNA, and male-specific fragments were identified. Further hybridization of male-specific clones to female XX Y, and male X 0 DNAs established them as containing Y-specific moderately repeated sequences. Further verification of one particular cloned fragment as Y-specific is presented and possible applications of this procedure are briefly discussed. © 1979.