GENOTYPIC IDENTIFICATION OF PATHOGENIC MYCOBACTERIUM SPECIES BY USING A NONRADIOACTIVE OLIGONUCLEOTIDE PROBE

被引:39
作者
LIM, SD [1 ]
TODD, J [1 ]
LOPEZ, J [1 ]
FORD, E [1 ]
JANDA, JM [1 ]
机构
[1] CALIF DEPT HLTH SERV,MICROBIAL DIS LAB,BERKELEY,CA 94704
关键词
D O I
10.1128/JCM.29.6.1276-1278.1991
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Commercial DNA hybridization assays (Syngene, Inc., San Diego, Calif.) utilizing alkaline phosphatase-labeled oligonucleotide probes for the identification of Mycobacterium tuberculosis complex and M. avium complex (MAC) were evaluated with 261 isolates of mycobacteria. On the basis of biochemical criteria, the test for MAC was 98% specific and more sensitive (95 of 99, 95%) than Gen-Probe (88 of 99, 89% sensitivity); the major difference in sensitivity noted between the two systems was related to the hybridization of seven MAC strains to the SNAP X probe. The M. tuberculosis complex probe correctly identified all 62 isolates of M. tuberculosis and all 11 isolates of M. bovis, for a sensitivity of 100%. There were two discrepant reactions with mycobacteria other than M. tuberculosis complex isolates.
引用
收藏
页码:1276 / 1278
页数:3
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