PURIFICATION AND CHARACTERIZATION OF THE DIPHTHERIA-TOXIN REPRESSOR

被引：65

作者：

SCHMITT, MP ^{[1
]}

TWIDDY, EM ^{[1
]}

HOLMES, RK ^{[1
]}

机构：

[1] UNIFORMED SERV UNIV HLTH SCI,DEPT MICROBIOL,BETHESDA,MD 20814

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 16期

关键词：

D O I：

10.1073/pnas.89.16.7576

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The diphtheria toxin repressor gene (dtxR) encodes a protein (DtxR) that regulates transcription of the diphtheria toxin gene (tox) by an iron-dependent mechanism. Cloned dtxR was expressed in Escherichia coli from the phage T7 gene 10 promoter, and DtxR was purified. Specific binding of DtxR to the tox+ operator was dependent on reduction of DtxR and the presence of ferrous ions. DtxR protected a sequence of almost-equal-to 30 nucleotide pairs, partially overlapping the tox promoter and containing a region of dyad symmetry, from digestion by DNase I. DtxR exhibited very little binding to the mutant tox-201 operator region and failed to bind to the promoter/operator region of the ferric uptake regulation (fur) gene of E. coli.

引用

页码：7576 / 7580

页数：5

共 40 条

[1] MOLECULAR MECHANISM OF REGULATION OF SIDEROPHORE-MEDIATED IRON ASSIMILATION [J].

BAGG, A ;

NEILANDS, JB .

MICROBIOLOGICAL REVIEWS, 1987, 51 (04) :509-518

[2]

BJORN MJ, 1978, INFECT IMMUN, V19, P785, DOI 10.1128/IAI.19.3.785-791.1978

[3] MOLECULAR-CLONING AND DNA-SEQUENCE ANALYSIS OF A DIPHTHERIA TOX IRON-DEPENDENT REGULATORY ELEMENT (DTXR) FROM CORYNEBACTERIUM-DIPHTHERIAE [J].

BOYD, J ;

OZA, MN ;

MURPHY, JR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (15) :5968-5972

[4] ANALYSIS OF THE DIPHTHERIA TOX PROMOTER BY SITE-DIRECTED MUTAGENESIS [J].

BOYD, J ;

MURPHY, JR .

JOURNAL OF BACTERIOLOGY, 1988, 170 (12) :5949-5952

[5] ISOLATION AND CHARACTERIZATION OF HYPERTOXINOGENIC (HTX) MUTANTS OF ESCHERICHIA-COLI KL320(PCG86) [J].

BRAMUCCI, MG ;

TWIDDY, EM ;

BAINE, WB ;

HOLMES, RK .

INFECTION AND IMMUNITY, 1981, 32 (03) :1034-1044

[6]

BRAUN V, 1985, TRENDS BIOCHEM SCI, V10, P76

[7] IRON REGULATION OF SHIGA-LIKE TOXIN EXPRESSION IN ESCHERICHIA-COLI IS MEDIATED BY THE FUR LOCUS [J].

CALDERWOOD, SB ;

MEKALANOS, JJ .

JOURNAL OF BACTERIOLOGY, 1987, 169 (10) :4759-4764

[8] REGULATION OF TOXINOGENESIS IN CORYNEBACTERIUM-DIPHTHERIAE - MUTATIONS IN THE BACTERIAL GENOME THAT ALTER THE EFFECTS OF IRON ON TOXIN PRODUCTION [J].

CRYZ, SJ ;

RUSSELL, LM ;

HOLMES, RK .

JOURNAL OF BACTERIOLOGY, 1983, 154 (01) :245-252

[9] OPERATOR SEQUENCES OF THE AEROBACTIN OPERON OF PLASMID-COLV-K30 BINDING THE FERRIC UPTAKE REGULATION (FUR) REPRESSOR [J].

DELORENZO, V ;

WEE, S ;

HERRERO, M ;

NEILANDS, JB .

JOURNAL OF BACTERIOLOGY, 1987, 169 (06) :2624-2630

[10]

DELORENZO V, 1988, EUR J BIOCHEM, V173, P537

← 1 2 3 4 →