IMPROVED SYNTHESIS OF 6-[P-(BROMOACETAMIDO)BENZYL]-1,4,8,11-TETRAAZACYCLOTETRADECANE-N,N',N'',N'''-TETRAACETIC ACID AND DEVELOPMENT OF A THIN-LAYER ASSAY FOR THIOL-REACTIVE BIFUNCTIONAL CHELATING-AGENTS

被引：27

作者：

MORAN, JK ^{[1
]}

GREINER, DP ^{[1
]}

MEARES, CF ^{[1
]}

机构：

[1] UNIV CALIF DAVIS, DEPT CHEM, DAVIS, CA 95616 USA

来源：

BIOCONJUGATE CHEMISTRY | 1995年 / 6卷 / 03期

关键词：

D O I：

10.1021/bc00033a010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Monoclonal antibodies labeled with radiometals such as copper-67 have applications in radioimmunodiagnosis and radioimmunotherapy. Moi et al. [(1985) Anal. Biochem. 148, 249-253] showed that 6-[p-(bromoacetamido)benzyl]-1,4,8,11-tetraazacyclotetradecane-N,N',N '',N'''-tetraacetic acid (BAT) is an effective reagent for linking copper to proteins, and antibodies labeled with copper radionuclides are currently undergoing clinical trials. Here we describe improvements in the original synthesis that increase the overall yield of BAT to 23%. We also describe a new assay useful to determine the activity of bifunctional chelating agents with thiol-reactive functional groups.

引用

页码：296 / 301

页数：6

共 22 条

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