VARIATION OF THE HEPATITIS-C VIRUS 5' NONCODING REGION - IMPLICATIONS FOR SECONDARY STRUCTURE, VIRUS DETECTION AND TYPING

被引：188

作者：

SMITH, DB

MELLOR, J

JARVIS, LM

DAVIDSON, F

KOLBERG, J

URDEA, M

YAP, PL

SIMMONDS, P

CONRADIE, JD

NEILL, AGS

DUSHEIKO, GM

KEW, MC

CROOKES, R

KOSHY, A

LIN, CK

LAI, C

MURRAYLYON, IM

ELGUNEID, A

GUNAID, AA

YEMEN, T

YEMEN, S

MUTIMER, D

AHMED, M

NUCHPRAYOON, C

TANPRASERT, S

PRESTON, FE

MAKRIS, M

CHUANSUMRIT, A

MAHASANDANA, C

PRITCHARD, D

RILEY, E

GREENWOOD, BM

SAEED, AA

ALRASHEED, AM

SALEH, MG

MCFARLANE, I

TIBBS, C

WILLIAMS, R

POWER, J

LAWLOR, E

KIYOKAWA, H

机构：

[1] EDINBURGH & SE SCOTLAND BLOOD TRANSFUS SERV, EDINBURGH EH3 9HB, MIDLOTHIAN, SCOTLAND

[2] CHIRON CORP, EMERYVILLE, CA 94608 USA

[3] NATAL INST IMMUNOL & BLOOD TRANSFUS SERV, DURBAN, SOUTH AFRICA

[4] ROYAL FREE HOSP, LONDON, ENGLAND

[5] UNIV WITWATERSRAND, JOHANNESBURG, SOUTH AFRICA

[6] BLOOD TRANSFUS SERV, JOHANNESBURG, SOUTH AFRICA

[7] KUWAIT UNIV, KUWAIT, KUWAIT

[8] RED CROSS BLOOD TRANSFUS SERV, YAUMATEI, HONG KONG

[9] CHARING CROSS HOSP, LONDON, ENGLAND

[10] AL THOWRA HOSP, TAIZI, YEMEN

[11] QUEEN ELIZABETH HOSP, BIRMINGHAM, W MIDLANDS, ENGLAND

[12] RED CROSS SOC, BANGKOK, THAILAND

[13] ROYAL HALLAMSHIRE HOSP, SHEFFIELD, S YORKSHIRE, ENGLAND

[14] RAMATHIBODI HOSP, BANGKOK, THAILAND

[15] SIRIRAJ HOSP, BANGKOK, THAILAND

[16] UNIV NOTTINGHAM, NOTTINGHAM, ENGLAND

[17] MRC LABS, Fajara, SENEGAL

[18] RIYADH ARMED FORCES HOSP, RIYADH, SAUDI ARABIA

[19] UNIV LONDON KINGS COLL HOSP, LONDON, ENGLAND

[20] BLOOD TRANSFUS SERV BOARD, DUBLIN, IRELAND

[21] RED CROSS BLOOD CTR, FUKUOKA, JAPAN

[22] AL THOWRA HOSP, SANAA, YEMEN

来源：

JOURNAL OF GENERAL VIROLOGY | 1995年 / 76卷

基金：

英国惠康基金;

关键词：

D O I：

10.1099/0022-1317-76-7-1749

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Variation in the 5' non-coding region (5'NCR) of hepatitis C virus (HCV) was investigated in detail by comparing 314 5'NCR sequences of viruses of genotypes 1 to 6. Evidence was obtained for the existence of associations between particular 5'NCR sequence moths and virus types and subtypes. No recombination was observed between the 5'NCR and coding regions of different genotypes, implying that the sequence of subgenomic regions such as the 5'NCR can be used to deduce virus genotype. The distribution of polymorphic sites within the 5'NCR is used to propose improved oligonucleotide primers for virus detection and quantification that would be equally efficient in detecting RNA of different virus genotypes. The accuracy of two different genotyping methods (RFLP and the line probe assay) based on analysis of sequence polymorphisms in the 5'NCR is predicted from the sequences surveyed to be 97% and 83% respectively for types 1 to 6, with higher accuracies for distinguishing between subtypes 1a/1b, 2a/2b or 3a/3b. Several sites of genotype-specific polymorphism were covariant and maintained the base pairings required for a secondary structure model of the 5'NCR. Other sites of variation suggest minor modifications to this model and have implications for the probable functions of the 5'NCR

引用

页码：1749 / 1761

页数：13