RECONSTITUTION OF OXIDATIVE PHOSPHORYLATION IN MYCOBACTERIUM PHLEI WITH CIS- AND TRANS-PHYLLOQUINONE . EVIDENCE AGAINST ISOMERIZATION

被引：14

作者：

DIMARI, SJ

RAPOPORT, H

机构：

[1] Department of Chemistry, University of California, Berkeley, California

来源：

BIOCHEMISTRY | 1968年 / 7卷 / 07期

关键词：

D O I：

10.1021/bi00847a030

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interconversion of cis- and trans-phyl-loquinones during oxidative phosphorylation by cell-free extracts from Mycobacterium phlei was studied using phylloquinone-5,6,7,8-t4. Both cis- and trans-phyl-loquinone restored oxidative phosphorylation to light-inactivated extracts, but the cis isomer did so to a distinctly lesser extent. Recovered quinone from test systems to which trans-phylloquinone had been added showed no evidence of isomerization. Test systems to which cis-phylloquinone had been added did lead to a small fraction of trans isomer in the recovered quinone, but this was due to experimental manipulations and not enzymic action. In particular, ordinary laboratory light caused cis-trans interconversions. © 1968, American Chemical Society. All rights reserved.

引用

页码：2650 / &

共 3 条

[1] ROLE OF QUINONE IN OXIDATIVE PHOSPHORYLATION . EVIDENCE AGAINST CARBON-HYDROGEN BOND CLEAVAGE [J].