MEASUREMENT OF ENZYME-ACTIVITIES OF CYTOCHROME-P-450 ISOENZYMES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF PRODUCTS

被引：40

作者：

REINERINK, EJM ^{[1
]}

DOORN, L ^{[1
]}

JANSEN, EHJM ^{[1
]}

VANIERSEL, AAJ ^{[1
]}

机构：

[1] NATL INST PUBL HLTH & ENVIRONM PROTECT, TOXICOL LAB, POB 1, 3720 BA BILTHOVEN, NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 553卷 / 1-2期

关键词：

D O I：

10.1016/S0021-9673(01)88493-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method is described for the qualitative and quantitative determinations of the isoenzymes of cytochrome P-450 from rat liver microsomes. Microsomes are incubated with the endogenous steroid 17-beta-testosterone, which results in the formation of a number of stereo-specific hydroxylation products of testosterone. The hydroxylated products were identified using standards or by comparison with data from the literature. The products can be analysed by reversed-phase gradient high-performance liquid chromatography. The assay has been optimised for pH, linearity and time of incubation. An evaluation of the assay was performed for different kinds of microsomes, microsomal dilution and specificity for particular cytochrome P-450 isoenzymes.

引用

页码：233 / 241

页数：9

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