NONRADIOACTIVE, SEQUENCE-SPECIFIC DETECTION OF RNA INSITU BY PRIMED INSITU LABELING (PRINS)

被引：30

作者：

MOGENSEN, J ^{[1
]}

KOLVRAA, S ^{[1
]}

HINDKJAER, J ^{[1
]}

PETERSEN, S ^{[1
]}

KOCH, J ^{[1
]}

NYGARD, M ^{[1
]}

JENSEN, T ^{[1
]}

GREGERSEN, N ^{[1
]}

JUNKER, S ^{[1
]}

BOLUND, L ^{[1
]}

机构：

[1] SKEJBY SYGEHUS & AARHUS KOMMUNEHOSP,DEPT CLIN CHEM,MOLEC GENET LAB,AARHUS,DENMARK

来源：

EXPERIMENTAL CELL RESEARCH | 1991年 / 196卷 / 01期

关键词：

MESSENGER-RNAS; HYBRIDIZATION; DNA; TRANSCRIPTION; EXPRESSION; PROBES; CELLS; GENE;

D O I：

10.1016/0014-4827(91)90459-8

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A new method for the detection of RNA in situ is presented. It is based on sequence-dependent annealing of unlabeled specific oligonucleotide primers to intracellular RNA and subsequent chain elongation catalyzed by reverse transcriptase. Under the conditions described, biotin-labeled nucleotides can be incorporated and the cDNA synthesized in situ can thus be detected using fluorescein-conjugated avidin. Compared to traditional in situ hybridization the use of short oligonucleotide primers has the potential advantage of being better to discriminate between closely related RNA transcripts. Compared to in situ transcription with radioactive precursors we find it more attractive to use fluorescein-conjugated avidin as detection system because it allows a more detailed study of cell and signal simultaneously. © 1991.

引用

页码：92 / 98

页数：7

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