ISOLATION AND CHARACTERIZATION OF A CDNA-ENCODING A 3-HYDROXY-3-METHYLGLUTARYL COENZYME-A REDUCTASE FROM NICOTIANA-SYLVESTRIS

被引：53

作者：

GENSCHIK, P ^{[1
]}

CRIQUI, MC ^{[1
]}

PARMENTIER, Y ^{[1
]}

MARBACH, J ^{[1
]}

DURR, A ^{[1
]}

FLECK, J ^{[1
]}

JAMET, E ^{[1
]}

机构：

[1] CNRS,INST BIOL MOLEC PLANTES,12 RUE GEN ZIMMER,F-67000 STRASBOURG,FRANCE

来源：

PLANT MOLECULAR BIOLOGY | 1992年 / 20卷 / 02期

关键词：

HMG-COA REDUCTASE; ISOPRENOID BIOSYNTHESIS; NICOTIANA-SYLVESTRIS; PROTOPLAST; STRESS;

D O I：

10.1007/BF00014504

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA library from freshly isolated mesophyll protoplasts of Nicotiana sylvestris was differentially screened using cDNAs from leaves, leaf strips submitted to the same stress as protoplasts during the isolation procedure, and cell suspension cultures. One of the selected clones (6P2) was found to encode a putative polypeptide highly homologous to previously characterized 3-hydroxy-3-methylglutaryl coenzyme A reductases. The C-terminal region of the polypeptide was highly conserved whereas its N-terminal region including the trans-membrane domains and the linker was more variable. Apart from protoplasts, the 6P2 gene was found to be expressed in apexes, anthers, roots, and in stressed leaf strips after 24 h of culture, during the hypersensitive reaction to viral infection and after HgCl2 treatment. This pattern of expression is consistent with a role in plant defence mechanisms.

引用

页码：337 / 341

页数：5

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