Isolation and Molecular Characterization of a Highly Polymorphic Centromeric Tandem Repeat in the Family Falconidae

被引:256
作者
Longmire, Jonathan L. [1 ]
Lewis, Annette K. [1 ]
Brown, Nancy C. [1 ]
Buckingham, Judith M. [1 ]
Clark, Lynn M. [1 ]
Jones, Myrna D. [1 ]
Meincke, Linda J. [1 ]
Meyne, Julianne [1 ]
Ratliff, Robert L. [1 ]
Ray, F. Andrew [1 ]
Wagner, Robert P. [1 ]
Moyzis, Robert K. [1 ]
机构
[1] Univ Calif Los Alamos Natl Lab, Div Life Sci, Los Alamos, NM 87545 USA
关键词
D O I
10.1016/0888-7543(88)90104-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
An abundant tandem repeat has been cloned from genomic DNA of the merlin (Falco columbarius). The cloned sequence is 174 bp in length, and maps by in situ hybridization to the centromeric regions of several of the large chromosomes within the merlin karyotype. Complementary sequences have been identified within a variety of falcon species; these sequences are either absent or in very low copy number in the family Accipitridae. The cloned merlin repeat reveals highly polymorphic restriction patterns in the peregrine falcon (Falco peregrinus). These polymorphisms, which have been shown to be stably inherited within a family of captive. peregrines, can be used to differentiate the Greenland and Argentina populations of this endangered raptor species. (C) 1988 Academic Press, Inc.
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页码:14 / 24
页数:11
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