AUTOMATED SAMPLE CLEANUP WITH SOLID-PHASE EXTRACTION FOR THE DETERMINATION OF AFLATOXINS IN URINE BY LIQUID-CHROMATOGRAPHY

被引：21

作者：

KUSSAK, A ^{[1
]}

ANDERSSON, B ^{[1
]}

ANDERSSON, K ^{[1
]}

机构：

[1] NATL INST OCCUPAT HLTH,DIV ANALYT CHEM,POB 7654,S-90713 UMEA,SWEDEN

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 616卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)80391-G

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An automated extraction and clean-up procedure was developed for the determination of aflatoxins in human urine at the 50 Pg/ml level. Aflatoxins B1, B2, G1 and G2 are captured on C2 extraction columns and simultaneously cleaned up with the aid of a robotic system. The processed samples are analysed by reversed-phase high-performance liquid chromatography. Fluorescence detection was enhanced for aflatoxins B1 and G1 using factorial design optimization of the post-column reactor. Silylation of the glass vials used in the robotic system was of the utmost importance. With non-silylated glass vials, up to 75% of the analytes were lost. Average aflatoxin recoveries were B1 95% B2 90%, G1 93% and G2 89%.

引用

页码：235 / 241

页数：7

共 22 条

[1] EXPOSURE TO AFLATOXINS IN DENMARK [J].

AUTRUP, H .

JOURNAL OF TOXICOLOGY-TOXIN REVIEWS, 1989, 8 (1-2) :53-67

[2] AFLATOXIN METABOLITES IN THE URINE OF NIGERIANS COMPARISON OF CHROMATOGRAPHIC METHODS [J].

BEAN, TA ;

YOURTEE, DM ;

AKANDE, B ;

OGUNLEWE, J .

JOURNAL OF TOXICOLOGY-TOXIN REVIEWS, 1989, 8 (1-2) :43-52

[3]

BETINA V, 1989, MYCOTOXINS BIOACTIVE, V9

[4]

CARLSON R, 1992, DESIGN OPTIMIZATION

[5] SUBSTANCES WITH AFFINITY TO A MONOCLONAL AFLATOXIN-B1 ANTIBODY IN DANISH URINE SAMPLES [J].

DRAGSTED, LO ;

BULL, I ;

AUTRUP, H .

FOOD AND CHEMICAL TOXICOLOGY, 1988, 26 (03) :233-242

[6]

Dvorackova I., 1990, AFLATOXINS HUMAN HLT

[7] DETERMINATION OF AFLATOXIN-M1 USING A DIALYSIS-BASED IMMUNOAFFINITY SAMPLE PRETREATMENT SYSTEM COUPLED ONLINE TO LIQUID-CHROMATOGRAPHY - REUSABLE IMMUNOAFFINITY COLUMNS [J].

FARJAM, A ;

VANDEMERBEL, NC ;

NIEMAN, AA ;

LINGEMAN, H ;

BRINKMAN, UAT .

JOURNAL OF CHROMATOGRAPHY, 1992, 589 (1-2) :141-149

[8] DETERMINATION OF AFLATOXINS IN PEANUT PRODUCTS USING DISPOSABLE BONDED-PHASE COLUMNS AND POSTCOLUMN REACTION DETECTION [J].

HURST, WJ ;

SNYDER, KP ;

MARTIN, RA .

JOURNAL OF CHROMATOGRAPHY, 1987, 409 :413-418

[9] PRIMARY LIVER-CANCER AND OCCUPATIONAL EXPOSURE [J].

KAUPPINEN, T ;

RIALA, R ;

SEITSAMO, J ;

HERNBERG, S .

SCANDINAVIAN JOURNAL OF WORK ENVIRONMENT & HEALTH, 1992, 18 (01) :18-25

[10]

KOELTZOW DE, 1990, J ASSOC OFF ANA CHEM, V73, P584

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