AUTORADIOGRAPHIC LOCALIZATION OF TRITIATED SURAMIN POLARIZED HUMAN COLON ADENOCARCINOMA CELLS

被引：9

作者：

BAGHDIGUIAN, S

BOUDIER, JA

BOUDIER, J

FANTINI, J

机构：

[1] FAC MED MARSEILLE,INSERM,U374,F-13916 MARSEILLE 20,FRANCE

[2] FAC MED MARSEILLE,INSERM,U270,F-13916 MARSEILLE 20,FRANCE

来源：

CANCER LETTERS | 1993年 / 75卷 / 03期

关键词：

HT-29-D4; CELLS; CANCER; SURAMIN; POLARITY; LYSOSOMAL DISORDER; QUANTITATIVE AUTORADIOGRAPHY;

D O I：

10.1016/0304-3835(93)90057-G

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

In this report we demonstrated that [H-3]suramin enters polarized human colon adenocarcinoma cells when added to the apical side of the monolayer. Using light microscopic quantitative autoradiography, we showed that suramin was accumulated in the apical eytoplasm and in the nucleus. In contrast, a weak labeling was noted in other compartments such as the basolateral cytoplasm and the intercellular space. The accumulation of suramin in the apical region of the cells is consistent with previous data showing that suramin elicited a lysosomal storage disorder in HT29-D4 cells by a mechanism of polarized endocytosis.

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收藏

页码：151 / 156

页数：6

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