EXPRESSION OF MESSENGER-RNA AND STEADY-STATE LEVELS OF PROTEIN ISOFORMS OF ENOYL-ACP REDUCTASE FROM BRASSICA-NAPUS

被引:36
作者
FAWCETT, T
SIMON, WJ
SWINHOE, R
SHANKLIN, J
NISHIDA, I
CHRISTIE, WW
SLABAS, AR
机构
[1] BROOKHAVEN NATL LAB,DEPT BIOL,UPTON,NY 11973
[2] NATL INST BASIC BIOL,OKAZAKI,AICHI 444,JAPAN
[3] SCOTTISH CROP RES INST,DUNDEE DD2 5DA,SCOTLAND
关键词
BRASSICA NAPUS; ENOYL-ACP REDUCTASE; ISOFORMS; STEAROYL-ACP DESATURASE; DEVELOPMENTAL EXPRESSION; SEED;
D O I
10.1007/BF00039528
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The expression of mRNA and the steady-state levels of two-component enzymes of plant fatty acid synthetase (FAS) were studied. Northern analysis of enoyl-ACP reductase (ER) and stearoyl-ACP desaturase (SD) gene expression showed that steady-state levels of both transcripts increase during lipid deposition in the seed reaching a maximum al 29 days after flowering (DAF). The steady-state level of ER message falls very quickly after reaching its maximum, whereas the SD message is longer-lived. The levels of these specific mRNAs in seed are 15-30 times greater than in leaf. Optimum mRNA expression precedes the maximum levels of synthesis of the two proteins, which in turn precede the maximum level of oil. The expression of isoenzymes of ER were examined by two-dimensional western blotting in both leaf and seed tissue. Four enzymes are expressed in both of these tissues; the two most abundant isoforms in seed material are also the most abundant in leaf tissue.
引用
收藏
页码:155 / 163
页数:9
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