NANOSECOND ELECTRON-TRANSFER KINETICS IN PHOTOSYSTEM-I FOLLOWING SUBSTITUTION OF QUINONES FOR VITAMIN-K1 AS STUDIED BY TIME RESOLVED EPR

被引：44

作者：

SIECKMAN, I ^{[1
]}

VANDEREST, A ^{[1
]}

BOTTIN, H ^{[1
]}

SETIF, P ^{[1
]}

STEHLIK, D ^{[1
]}

机构：

[1] CENS,DEPT BIOL,SERV BIOPHYS,F-91191 GIF SUR YVETTE,FRANCE

来源：

FEBS LETTERS | 1991年 / 284卷 / 01期

关键词：

PHOTOSYSTEM-I; VITAMIN-K1; ELECTRON TRANSFER; TIME RESOLVED EPR; A1 EXTRACTION RECONSTITUTION; SYNECHOCYSTIS; 6803;

D O I：

10.1016/0014-5793(91)80771-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Room temperature transient EPR spectra of photosystem I (PS I) particles from Synechocystis 6803 are presented. Native PS I samples and preparations depleted in the A1-acceptor site by solvent extraction and then reconstituted with the quinones (Q) vitamin K1 (VK1), duroquinone (DQ and DQd12) and naphthoquinone (NQ) have been studied. Sequential electron transfer to P700+ A1- (FeS) and P700+ A1- (FeS)- is recovered only with VK1. With DQ and NQ electron transfer is restored to form the radical pair P700+ Q- as specified by a characteristic electron spin polarization (ESP)-pattern, but further electron transfer is either slowed down or blocked. A qualitative analysis of the K-band spectrum suggests that the orientation of reconstituted NQ in PS I is different from the native acceptor A1 = VK1.

引用

页码：98 / 102

页数：5

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