STRUCTURE AND EXPRESSION OF THE BARLEY LIPID TRANSFER PROTEIN GENE LTP1

被引：62

作者：

SKRIVER, K ^{[1
]}

LEAH, R ^{[1
]}

MULLERURI, F ^{[1
]}

OLSEN, FL ^{[1
]}

MUNDY, J ^{[1
]}

机构：

[1] CARLSBERG RES LAB,GL CARLSBERG VEJ 10,DK-2500 COPENHAGEN,DENMARK

来源：

PLANT MOLECULAR BIOLOGY | 1992年 / 18卷 / 03期

关键词：

ALEURONE LAYER; ENZYME INHIBITOR; GENE SEQUENCE; TRANSIENT EXPRESSION;

D O I：

10.1007/BF00040674

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of Ltp1 showed that it contains an open reading frame of 351 bp interrupted by a single intron of 133 bp. Transient expression assays indicated that 702 bp of the 5' upstream region of Ltp1 is sufficient to direct aleurone-specific expression during late seed development and early germination.

引用

页码：585 / 589

页数：5

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