PHOTOPHYSICS IN THE PICOSECOND TIME RANGE OF THE METHYLENE-BLUE GUANOSINE-5'-MONOPHOSPHATE COMPLEX

被引：11

作者：

ENESCU, M

KRIM, L

LINDQVIST, L

WU, TQ

机构：

[1] Laboratoire de Photophysique Moléculaire, CNRS, Université Paris-Sud, 91405 Orsay

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 1994年 / 22卷 / 02期

关键词：

METHYLENE BLUE-GUANOSINE COMPLEX; PICOSECOND SPECTROSCOPY; DEACTIVATION PATHWAYS;

D O I：

10.1016/1011-1344(93)06971-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complex of methylene blue with guanosine-5'-monophosphate was studied by transient absorption laser spectroscopy with 1 ps resolution. The prompt transient spectrum was found to be similar to that of the free dye, while the excited-state lifetime is strongly reduced. Identical decays for S-1-->S-n absorption, ground-state bleaching and stimulated emission were obtained. The biexponential decay (lifetimes 8 and 160 ps, respectively) was found to be associated with time-independent shapes of these transient spectra. We conclude that the observed lifetime shortening is due to the enhancement of non-radiative transitions between S-1 and S-0 by involvement of a low-lying charge transfer state, rather than to an electron transfer process with complete charge separation.

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页码：165 / 169

页数：5

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