NEURAL ANTIGEN-DETECTION IN MOUSE-TISSUES IS NOT IMPAIRED BY DECALCIFICATION

被引:6
作者
HENKEN, DB
MARTIN, JR
机构
[1] Laboratory of Experimental Neuropathology, National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, 20892, MD, Building 36
关键词
EDTA DECALCIFICATION; SENSORY GANGLIA; IMMUNOCYTOCHEMISTRY; CALCITONIN GENE-RELATED PEPTIDE; SUBSTANCE-P;
D O I
10.1007/BF00334885
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
We examined whether EDTA decalcification decreases the sensitivity of antigen detection of formalin-fixed paraffin-embedded neural tissues. In undissected decalcified tissues, the immunoreactivities of select neural antigens were quantitatively compared with those in dissected sensory ganglia. Decalcification does not reduce either the numbers or staining intensities of antigen-positive neural cells. Tissues are well preserved and complex anatomical relationships are maintained.
引用
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页码:176 / 178
页数:3
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