ENZYMES INVOLVED IN THEOBROMINE PRODUCTION FROM CAFFEINE BY PSEUDOMONAS-PUTIDA NO-352

被引：33

作者：

ASANO, Y ^{[1
]}

KOMEDA, T ^{[1
]}

YAMADA, H ^{[1
]}

机构：

[1] KYOTO UNIV, DEPT AGR CHEM, KYOTO 606, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 12期

关键词：

D O I：

10.1271/bbb.58.2303

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A monooxygenase specific for theobromine demethylation was purified from the cell-free extract of Pseudomonas putida No. 352 to homogeneity. Caffeine appears to be demethylated to 7-methylxanthine at least by two enzymes, caffeine demethylase and theobromine demethylase. The latter is sensitive to Zn2+ and possibly caused an accumulation of theobromine in P. putida No. 352.

引用

页码：2303 / 2304

页数：2

共 5 条

[1] PURIFICATION AND PROPERTIES OF FORMATE DEHYDROGENASE FROM MORAXELLA SP STRAIN-C-1 [J].