IMMORTALIZED ANTERIOR-PITUITARY ALPHA-T3 GONADOTROPES CONTAIN FUNCTIONAL LUTEINIZING-HORMONE HUMAN CHORIONIC-GONADOTROPIN RECEPTORS

被引：18

作者：

HUANG, ZH ^{[1
]}

LEI, ZM ^{[1
]}

RAO, CV ^{[1
]}

机构：

[1] UNIV LOUISVILLE, SCH MED, DEPT OBSTET & GYNECOL, LOUISVILLE, KY 40292 USA

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1995年 / 114卷 / 1-2期

关键词：

IMMORTALIZED ANTERIOR PITUITARY ALPHA T3 GONADOTROPES; HUMAN CHORIONIC GONADOTROPIN (HCG);

D O I：

10.1016/0303-7207(95)03635-K

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Immortalized alpha T3 gonadotropes contain a major 5.0-kb and minor 3.6-, 2.4- and 1.6-kb luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor transcripts and an 80-kDa receptor protein which can specifically bind I-125-hCG. The relative abundance of the receptors in (alpha T3 cells is, however, lower than in gonadal tissues. Culturing alpha T3 cells with highly purified hCG resulted in a dose- and time-dependent and hormone-specific increase in steady-state levels of LH-alpha subunit mRNA. Culturing (alpha T3 cells with hCG also resulted in a concomitant increase in the LH-alpha protein levels. As expected, culturing with GnRH also resulted in an increase of LH-alpha subunit mRNA and protein levels. In summary, we conclude that alpha T3 cells contain LH/hCG receptors which are functionally coupled to the up-regulation of LH-alpha subunit gene expression.

引用

页码：217 / 222

页数：6

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