EXTRACELLULAR ATP4- PERMEABILIZES THYMOCYTES NOT ONLY TO CATIONS BUT ALSO TO LOW-MOLECULAR-WEIGHT SOLUTES

被引:20
作者
ELMOATASSIM, C [1 ]
MANI, JC [1 ]
DORNAND, J [1 ]
机构
[1] FAC PHARM MONTPELLIER,IMMUNOL LAB,F-34060 MONTPELLIER,FRANCE
关键词
(Mouse); ATP[!sup]4-[!/sup; Cation entry; EL-4 cell line; Membrane permeabilization; Thymocytes;
D O I
10.1016/0014-2999(90)90251-Z
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Extracellular ATP increases the free cytosolic calcium concentration ([Ca2+]i) of thymocytes by changing the membrane permeability to divalent cations: (1) no [Ca2+]i elevationis observed in the absence of extracellular calcium and (2) ATP also promotes Mn2+ entry into thymocytes, as shown by a stimulated quench of the fluorescence of Quin2-loaded cells. Using 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene, a dye which usually does not pass through membranes, we showed that ATP permeabilizes thymocyte membranes not only to cations but also to low-molecular-weight solutes. The ATP effect is dose-dependent, specific and reversible; ATP4- appears to be the active species and probably acts through specific receptor(s). Both medullary and cortical thymocytes respond to ATP whereas splenocytes do not; this shows that the ATP effect is restricted to certain populations of lymphocytes. The thymoma-derived cell line, EL-4, the permeability of which is also sensitive to ATP, could provide a useful model for studying the putative ATP4- receptor(s) of thymocytes. © 1990.
引用
收藏
页码:111 / 118
页数:8
相关论文
共 27 条
  • [1] EFFECT OF EXOGENOUS ATP UPON INOSITOL PHOSPHATE PRODUCTION, CATIONIC FLUXES AND INSULIN RELEASE IN PANCREATIC-ISLET CELLS
    BLACHIER, F
    MALAISSE, WJ
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 970 (02) : 222 - 229
  • [2] LYMPHOCYTE MEMBRANE ADENOSINE RECEPTORS COUPLED TO ADENYLATE-CYCLASE PROPERTIES AND OCCURRENCE IN VARIOUS LYMPHOCYTE SUBCLASSES
    BONNAFOUS, JC
    DORNAND, J
    FAVERO, J
    MANI, JC
    [J]. JOURNAL OF RECEPTOR RESEARCH, 1981, 2 (04): : 347 - 366
  • [3] BOYER JL, 1989, J BIOL CHEM, V264, P884
  • [4] EXTRACELLULAR ATP INDUCES A LARGE NONSELECTIVE CONDUCTANCE IN MACROPHAGE PLASMA-MEMBRANES
    BUISMAN, HP
    STEINBERG, TH
    FISCHBARG, J
    SILVERSTEIN, SC
    VOGELZANG, SA
    INCE, C
    YPEY, DL
    LEIJH, PCJ
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (21) : 7988 - 7992
  • [5] BURNSTOCK G, 1978, CELL MEMBRANE RECEPT, P107
  • [6] EXTRACELLULAR ADENOSINE-TRIPHOSPHATE ACTIVATES CALCIUM MOBILIZATION IN HUMAN PHAGOCYTIC LEUKOCYTES AND NEUTROPHIL MONOCYTE PROGENITOR CELLS
    COWEN, DS
    LAZARUS, HM
    SHURIN, SB
    STOLL, SE
    DUBYAK, GR
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1989, 83 (05) : 1651 - 1660
  • [7] 5'-NUCLEOTIDASE-ADENYLATE CYCLASE RELATIONSHIPS IN MOUSE THYMOCYTES - RE-EVALUATION OF THE EFFECTS OF CONCANAVALIN-A ON CYCLIC-AMP LEVELS
    DORNAND, J
    BONNAFOUS, JC
    MANI, JC
    [J]. FEBS LETTERS, 1980, 110 (01) : 30 - 34
  • [8] DORNAND J, 1986, CELLULAR BIOL ECTOEN, P72
  • [9] DUBYAK GR, 1988, J BIOL CHEM, V263, P18108
  • [10] EXTRACELLULAR ATP INDUCES A NONSPECIFIC PERMEABILITY OF THYMOCYTE PLASMA-MEMBRANES
    ELMOATASSIM, C
    BERNAD, N
    MANI, JC
    DORNAND, J
    [J]. BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, 1989, 67 (09): : 495 - 502