THE PROTOTYPICAL EPSTEIN-BARR VIRUS-TRANSFORMED LYMPHOBLASTOID CELL-LINE IB4 IS AN UNUSUAL VARIANT CONTAINING INTEGRATED BUT NO EPISOMAL VIRAL-DNA

被引:61
作者
HURLEY, EA
KLAMAN, LD
AGGER, S
LAWRENCE, JB
THORLEYLAWSON, DA
机构
[1] TUFTS UNIV,SCH MED,DEPT PATHOL,136 HARRISON AVE,BOSTON,MA 02111
[2] UNIV MASSACHUSETTS,MED CTR,DEPT CELL BIOL,WORCESTER,MA 01605
关键词
D O I
10.1128/JVI.65.7.3958-3963.1991
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
IB4 is a prototype, latently Epstein-Barr virus (EBV)-infected, lymphoblastoid cell line. We show here that IB4 contains only integrated EBV genomes. Epsiomal EBV DNA is not detected by Gardella gel analysis or in situ hybridization. Restriction enzyme mapping indicates that the EBV genomes first circularized and then integrated into and deleted part of the BamHI C fragment. IB4 is therefore the only lymphoblastoid cell line described to date that lacks episomal EBV and has integrated EBV genomes with joined ends. Thus, the detection of joined EBV termini on Southern blots is not as reliable as the Gardella gel system for detecting episomal EBV DNA, and IB4 is not an ideal prototype cell line for the study of latent infection by EBV.
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收藏
页码:3958 / 3963
页数:6
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